The only Blues bar I know of is BB King's in Times Sq. However, many of the cabaret's will have performers who do blues. I would check the NY Times (Friday or Sunday) or the free tourist magazines.
Finding 'real' blues in NYC is like looking for Clam Chowder in Memphis or real New Orleans Jazz in Peoria. To find the blues and its music, you are required to come to the area described as the Mississippi Delta, where the blues wuz borned. The MS Delta is a geographic area beginning at the lobby of the Peabody Hotel in Memphis, TN, extending to Vicksburg, Mississippi, bounded on the west by the Mississippi River and on the East by the foothills of the Yazoo River.
You might find him at that place one or two nights out of 365. Of course he knows the blues. He was born and grew up in Indianola, MS, just 29 miles from where I grew up. I see him probably 5 times per year. He will sometimes show up at this place on Beale St. in Memphis, but largely, those places are there for commercial purposes. Hopefully he has some fairly good blues guys appearing on occasion. Yes, BB is the man, of those left alive. I saw him live, last about two months ago and will see him again in May at his annual Indianola homecoming.
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lends itself to tourists. Birdland is pretty famous too.
I asked my former college room-mate that works in NYC....this is her suggestion....